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KMID : 0380020140290060432
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 6 p.432 ~ p.436
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Antioxidative Activity of Extract of Cornus walteri Wanger Leaves in Human Dermal Fibroblast Irradiated by UVB
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Park Hyun-Chul
Jung Taek-Kyu
Yoon Kyung-Sup
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Abstract
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Abstract: We investigated antioxidative activity of the ethanol extracts of leaves of Cornus walteri Wanger (CWE) by treated enzyme in human dermal fibroblast (HDFs) irradiated by UVB. We examined the in vitro chemical and cellular antioxidant activities of CWE in HDFs. We employed scavenging assay for the 1,1-diphenyl-2,5-picrylhydrazyl (DPPH) radicals and cellular antioxidative activity of CWE, and we was investigated in H2O2-treated or UVB-irradiated HDFs. The CWE effectively scavenged DPPH radicals (IC50 7.03± 0.4 μg/mL) when compared to the scavenging activities of Lascorbic acid (IC50 4.69±0.3 μg/mL). CWE reduced UVBinduced cellular damage in HS68 cells by MTT assay and inhibited intracellular ROS generation in dose-dependent manner. In addition, CWE also attenuated the elevated levels of 8-isoprostane resulting from UVB-mediated oxidative stress. Collectively, these results suggest that CWE could be a new potential candidate as antioxidant against UVB-induced oxidative stress in HDFs.
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KEYWORD
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Cornus walteri Wanger, UVB-irradiation, Human dermal fibroblast (HDF), Antioxidative activity
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